Solution Equilibrium Titration for High-Throughput Affinity Estimation of Unpurified Antibodies and Antibody Fragments
نویسندگان
چکیده
منابع مشابه
Determination of equilibrium dissociation constants for recombinant antibodies by high-throughput affinity electrophoresis
High-quality immunoreagents enhance the performance and reproducibility of immunoassays and, in turn, the quality of both biological and clinical measurements. High quality recombinant immunoreagents are generated using antibody-phage display. One metric of antibody quality - the binding affinity - is quantified through the dissociation constant (KD) of each recombinant antibody and the target ...
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ژورنال
عنوان ژورنال: Journal of Biomolecular Screening
سال: 2015
ISSN: 1087-0571,1552-454X
DOI: 10.1177/1087057115595002